Question 1

Which of the following is not involved in gene cloning?

Accepted Answer

A)   host cell
B)   vector
C)   restriction enzyme
D)   dyes attached to nucleotides
E)   "sticky" endsF) A) and E)
G) A) and D)

Question 2

Which of the following is not a type of mRNA processing?

Accepted Answer

A)   adding a cap
B)   cleaving off the methionine
C)   adding a tail
D)   removing the introns
E)   splicingF) B) and C)
G) B) and D)

Question 3

One of the disadvantages of PCR is that it requires large amounts of starting material.

Accepted Answer

The answer of One of the disadvantages of PCR is...

Question 4

The bases in DNA are complementary. A always binds with

Accepted Answer

A)   A. 
B)   C.
C)   G.
D)   T.
E)   U.F) All of the above
G) None of the above

Question 5

In vivo gene therapy has been used to help patients suffering from cystic fibrosis.

Accepted Answer

A) True 
 B)False

Question 6

How does modern day sequencing detect dyes on the nucleotides?

Accepted Answer

A)   radioactivity
B)   dye terminator chemicals
C)   DNA breakage
D)   laser
E)   by eyeF) B) and D)
G) C) and E)

Question 7

Place the following steps in order for the cloning of a gene. 1. DNA ligase seals foreign DNA into a plasmid. 2.A restriction enzyme is used to cleave DNA. 3. The bacterium makes a product. 4. Some of the bacterial cells take up a recombinant plasmid. 5. The plasmid replicates on its own.

Accepted Answer

A)   2,1,4,5,3
B)   1,2,3,4,5
C)   3,1,2,4,5
D)   5,1,4,3,2
E)   1,3,2,4,5F) A) and B)
G) A) and C)

Question 8

Which of the following is not studied in the field of proteomics?

Accepted Answer

A)   protein-protein interactions
B)   protein concentration in a cell
C)   which genes give rise to which proteins
D)   cellular location of particular proteins
E)   chemical modifications of proteinsF) A) and B)
G) B) and D)

Question 9

In the RNA sequence CAAUGACCAG, what will be the first amino acid incorporated?

Accepted Answer

A)   alanine
B)   glutamine
C)   methionine
D)   valine
E)   glycineF) A) and D)
G) C) and D)

Question 10

Which of the following would be an example of pretranscriptional control?

Accepted Answer

A)   The chromosomes must decondense.
B)   Transcription factors help initiate transcription.
C)   The mRNA is processed before it leaves the nucleus.
D)   The life expectancy of the mRNA molecules vary.
E)   The polypeptide product is folded to form its functional conformation.F) All of the above
G) A) and E)

Question 11

Which of the following occurs during initiation of translation?

Accepted Answer

A)   The ribosome moves down one codon so that the A site is available to receive an incoming tRNA.
B)   The mRNA binds to the smaller of the two ribosomal subunits.
C)   The polypeptide lengthens one amino acid at a time.
D)   The ribosome dissociates into its two subunits and falls off the mRNA.
E)   One of the three stop codons is reached.F) C) and E)
G) C) and D)

Question 12

Which of the following nucleotide sequences could give rise to the amino acid sequence alanine-asparatate-glycine? Assume that translation initiation has already occurred.

Accepted Answer

A)   GUUAAGAGG
B)   GCCGACGGC
C)   CACCGCCGA
D)   GAUGGUGCU
E)   UUUUAAUGGF) D) and E)
G) A) and E)

Question 13

The denaturation of which enzyme would lead to incorrect base pairing of nucleotides during DNA replication?

Accepted Answer

A)   DNA polymerase
B)   DNA ligase
C)   DNA helicase
D)   binding proteins
E)   Okazaki fragmentsF) A) and B)
G) A) and E)

Question 14

DNA replication is considered

Accepted Answer

A)   conservative.
B)   semiconservative.
C)   dispersive.
D)   relaxed.
E)   stringent.F) None of the above
G) C) and E)

Question 15

If the codon is CCA, what is the anticodon and what amino acid will be inserted?

Accepted Answer

A)   CCA, proline
B)   GGU, proline
C)   GGT, glycine
D)   ACC, threonine
E)   UGG, tryptophanF) B) and C)
G) All of the above

Question 16

The 3-base sequence in a mRNA molecule is called a(n)

Accepted Answer

A)   codon.
B)   transcriptome.
C)   initiator.
D)   mutation.
E)   transposon.F) C) and E)
G) C) and D)

Question 17

List and explain the 5 steps involved in DNA replication.

Accepted Answer

1. The enzyme DNA helicase unwinds and u...

Question 18

In what way are DNA and RNA similar?

Accepted Answer

A)   Both contain C, A, and G. 
B)   Both contain deoxyribose.
C)   Both contain U, C, and G.
D)   Both are single stranded.
E)   Both form a double helix.F) All of the above
G) B) and C)

Question 19

How many genes did researchers think the human genome contained? How many did it contain?

Accepted Answer

A)   3 billion; 80,000
B)   1 million; 1 million
C)   25,000; 80,000
D)   80,000; 25,000
E)   125 million; 1 millionF) A) and B)
G) D) and E)

Question 20

What is the role of the enzyme ligase in DNA replication?

Accepted Answer

A)   It unzips the double-stranded DNA.
B)   It seals any breaks in the sugar-phosphate backbone.
C)   It breaks the hydrogen bonds that hold the two strands of the DNA together.
D)   It matches new bases to the old strand by complementary base pairing.
E)   It folds the DNA into a coiled structure.F) D) and E)
G) All of the above

Exam 21: DNA Biology and Technology

List and explain the 5 steps involved in DNA replication.

Correct Answerverified1. The enzyme DNA helicase unwinds and u...View AnswerShow Answer

Which of the following would be an example of pretranscriptional control?

Correct AnswerverifiedShow Answer

Place the following steps in order for the cloning of a gene. 1. DNA ligase seals foreign DNA into a plasmid. 2.A restriction enzyme is used to cleave DNA. 3. The bacterium makes a product. 4. Some of the bacterial cells take up a recombinant plasmid. 5. The plasmid replicates on its own.

Correct AnswerverifiedShow Answer

How does modern day sequencing detect dyes on the nucleotides?

Correct AnswerverifiedShow Answer

The 3-base sequence in a mRNA molecule is called a(n)

Correct AnswerverifiedShow Answer

DNA replication is considered

Correct AnswerverifiedShow Answer

Which of the following nucleotide sequences could give rise to the amino acid sequence alanine-asparatate-glycine? Assume that translation initiation has already occurred.

Correct AnswerverifiedShow Answer

The denaturation of which enzyme would lead to incorrect base pairing of nucleotides during DNA replication?

Correct AnswerverifiedShow Answer

How many genes did researchers think the human genome contained? How many did it contain?

Correct AnswerverifiedShow Answer

What is the role of the enzyme ligase in DNA replication?

Correct AnswerverifiedShow Answer

Which of the following occurs during initiation of translation?

Correct AnswerverifiedShow Answer

In vivo gene therapy has been used to help patients suffering from cystic fibrosis.

Correct AnswerverifiedShow Answer

The bases in DNA are complementary. A always binds with

Correct AnswerverifiedShow Answer

Which of the following is not involved in gene cloning?

Correct AnswerverifiedShow Answer

Which of the following is not studied in the field of proteomics?

Correct AnswerverifiedShow Answer

In what way are DNA and RNA similar?

Correct AnswerverifiedShow Answer

Which of the following is not a type of mRNA processing?

Correct AnswerverifiedShow Answer

If the codon is CCA, what is the anticodon and what amino acid will be inserted?

Correct AnswerverifiedShow Answer

One of the disadvantages of PCR is that it requires large amounts of starting material.

Correct AnswerverifiedShow Answer

In the RNA sequence CAAUGACCAG, what will be the first amino acid incorporated?

Correct AnswerverifiedShow Answer

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1. The enzyme DNA helicase unwinds and u...
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